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In mice heterozygous for Noggin gene deletion
Incubate at 37°C with 5% CO₂ for 24 hours
Co-IP Reactivity Species Independent Purification Protein A Conjugation Agarose beads Physical Appearance Liquid Storage Buffer 50% slurry in PBS pH7
Avoid introducing sodium azide (NaN) into the sample3)
Rat Bax ELISA Kit Size:96T In mice heterozygous for NogginProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
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